Equine Coat Color Genetics

 

Lesson Three

 

The Cream Dilution

Palomino, Buckskin, Smoky Brown, Smoky Black, Cremello, Perlino, Brown Cream & Smoky Cream

The Single & Double Cream Dilutes

          The term “dilute” is used to describe multiple colors: Cream, Champagne, Dun, Silver and Pearl.   In recent years, equine studies have shown that each of the colors are actually separate genes.  With the exception of the pearl gene, they reside on separate sections (alleles) of the chromosome.  In this section, we will address the Cream gene only.

          This is a good place to add two more words to our genetic vocabulary. The color of a horse determined by the way it looks is its phenotype.  The actual genetic code of the individual is its genotype.

 

          Determining the genotype has been described as "detective work". While there are laboratory tests available for most known color genes at this time, in the past, examination of the color of the ancestors and the colors produced from specific crosses, and tracing the genes from generation to generation, was the only way to ultimately determine the genotype of the horse in question.  However, almost all of the results obtained by the "old method" (which is still widely used)  have been confirmed by the lab tests now available.

 

A Word About "Albino"

 

          An albinos is by definition “A person or animal lacking normal coloring in the eyes, hair, and skin due to a hereditary inability to produce the skin pigment melanin. The condition itself is called albinism.” (http://medical-dictionary.thefreedictionary.com/albino).  Mice carry this gene, which in mice produces an all-white mouse with red eyes (from the blood vessels showing through the clear, unpigmented iris) and pink skin, completely devoid of any color pigment.  Studies have shown that the Equid species (all horses, mules, donkeys, asses, zebras, etc) do not carry the genetic mechanism to produce an albino.  Remember, "albino" in most animals denotes complete, total absence of color producing genetics.  So a horse with a white coat, pink skin & blue eyes is not a true "albino".  The effect described above can be produced by any of several combinations of color-modifying genes.  Most striking is that of a multi/maximum-pinto -- think of the horse being covered by so many white spots that there is no color left showing through anywhere -- produced by any number of pinto (aka paint) genes.

 

The Cream gene - Single and Double Dilutes

          The most common dilution gene, the cream color-modifying gene (Cr), is responsible for palomino, buckskin, smoky brown and smoky black colors, as well as what were (and sometimes still are) simply called “double dilutes” (cream dilution implied).   Cream is an incompletely dominant gene.  An easy way to think of the concept of “incomplete dominance” is to think of the gene providing only part of its capability to dilute when present in one copy, and its full capability when present in two copies.   In other words, an incomplete dominant expresses itself in a stronger form when two (2) copies are present (when homozygous).

          Because it is a type of dominant gene, we will use the abbreviation beginning with an upper-case letter : Cr.  The abbreviation C is not used because in other species, such as mice, it represents the absence of the albino gene (being, in those cases, the abbreviation for "color".)

          The absence of the cream gene, or not-cream, will be abbreviated cr.

Single and Double Cream Dilutes

          When present in one copy (heterozygous), the cream gene changes the red body color of sorrels/chestnuts and bays to a golden or tan color.  It changes a sorrel/chestnut to palomino, a bay horse to buckskin, a brown horse to a smoky brown (sometimes called a brown buckskin), and a solid black horse to a smoky black.  On a solid black horse, a single cream gene can be evinced by red or tawny highlights, or it may be virtually invisible.

          All of these single-cream-gene colors used to be called, simply, “single dilutes”, because cream was the only dilution gene known until recent years.

          When present in two (2) copies, or homozygous, the incomplete dominant intensifies and changes the single-dilute colors further.  Palominos become cremellos, buckskins become perlinos, smoky browns become brown creams, and smoky blacks become smoky creams.

          Some breeds of horses do not carry the cream gene (Cr).  The two which come to mind immediately are the Friesian and the modern Arabian.  In purebred horses of these breeds, one will never see palomino, buckskin, smoky brown, or smoky black animals.

          For a quick, fun introduction to the cream dilutes, see this web page: http://www.horsecolors.us/dilutions/cream/javatest2.htm

 

Palomino and Cremello

          In the heterozygous form (Crcr) this gene will change a sorrel/chestnut color to gold and the mane and tail to white.  This gives us Palomino. 

          Thus, the palomino horse is a chestnut horse (ee) with one copy of the dominant Cr gene. The genetic expression of this would be ee (any agouti) Crcr/

 

          The ideal shade of palomino is a body the color of a newly minted gold coin with a white mane and tail.  However, the body color of palominos can range from smoky gray to a yellow cream color. The manes and tails can be white or ivory or, in extremely rare cases, a shade close to or even darker than their body color.  (See "Sootiness")

 

                                                                 

 

          When the Cream gene is present in the homozygous form (CrCr) all of these coat colors lighten further.  One can compare it to putting one creamer or two in a cup of coffee.

 

          The palomino coat color will lighten one more step to cremello, and the normal brown eye will change to a blue eye as well.  The genetic expression of this “ee (any agouti) CrCr”.

 

 

 

                                                                                

                                                             Palomino Coloring                                                           Cremello Coloring

 

          The physical traits of a cremello are a cream colored body coat, a lighter/white colored mane and tail, a warm pink skin tone with some dark specks here and there, and blue to blue-green eyes.   Blue eyes on a double dilute are not the result of a blue-eyed gene, but of dilution of the iris pigment, and when one breeds a double cream to a non-cream the resulting (single-dilute) foal will not have blue eyes, unless the other parent carries the other genetics for blue eyes.

          The way most cremellos occurred in the past was through the mating of two palominos - ee (any agouti) Crcr.   This gives a 25% chance of sorrel/chestnut, 50% chance of palomino and 25% chance of producing a cremello.  Before cremellos were eligible for registration with the AQHA, that was the only way to breed for palomino in that breed, with a 25% chance of producing a "non-QH" from two pure QH parents!

 

 

 

 

 

          If one breeds a cremello to cremello one will always get a cremello.  The effect of a double dose of the cream gene is not magnified and there is no increase in the full effect of the cream gene in homozygous form.  Each parent gives one Cream gene to the offspring insuring the resulting foal inherits two cream genes.  Remember, the maximum number of genes involved are two (homozygous state) and the genes will never ‘magnify’ nor mutate when two double-dilute horses are bred together.

 

 

 

Buckskin and Perlino

          Buckskin is the result of one cream gene on a bay horse (black plus Agouti - E_A_).  The single cream gene changes the reddish body color of a bay horse to a golden or tan color, but does not affect the black mane, tail, ear tips, or legs of the horse. 

 

 

                                                                  

 

                                                        Typical Bay                                                                   Typical Buckskin

 

 

 

 

 

 

 

 

 

 

          When two cream genes are present, the black coloration of the mane and tail are lightened to a pale gold color and eyes are changed from normal brown to blue.  The most common way to produce Perlino is through the mating of two buckskins - E_A_Ccr.   This will give a 25% chance of a bay, 50% chance of a buckskin and 25% chance of a perlino.

 

 

                                                                    

 

                                                     Typical Buckskin                                                          Typical Perlino

 

 

 

 

 

 

 

 

 

 

Smoky Brown and Brown Cream

 

          Smoky brown is the result of one cream gene on a brown (E_ plus AtAt or Ata) horse.  Though some still refuse to accept the brown color group as extant, there are genetic tests as well as visible clues that differentiate it from black or bay.

A smoky brown may also be referred to as a brown buckskin.  This is a brown horse (see description in previous lesson) with one cream gene.  As one might expect, the result is somewhere between buckskin and smoky black.  Some simply call these "very sooty buckskins", but when the genetic testing is performed, the difference is clear.  They will have extensive darkness throughout the body when compared to a true, bay-based buckskin, yet golden-tan areas much more extensive than on a smoky black.  The points remain black.  The areas that are light on an undiluted brown horse are lighter on a smoky brown (muzzle, flanks, armpits, belly, and sometimes "eyebrows".)  The genotype for this color would be E_,  AtAt or Ata, Crcr.

 

 

 

 

DNA verified smoky brown

Photo from horsecolorforum

 

 

          When another cream gene is added, the resulting color is called brown cream, and looks much like a perlino, but usually with a slightly darker body color.  We have requested verified photos of these colors, which will be included as soon as they are received.

 

 

Smoky Black

 

          Smoky back is the result of one cream gene on a black horse.  The single cream gene does not dilute the black color of the horse in the same way it dilutes red body color.  Sometimes there will only be reddish or tawny highlights on the black body; sometimes the mane will "sunburn" to red much more quickly than the mane of a typical non-cream black; and sometimes it will seem impossible to detect, with the naked eye, that there is a cream gene present in the horse's make-up.

 

 

 

A black Friesian (no cream gene). 
(Many Friesians have a lot of brownish coloring, but they all genetically test pure, solid black -- no cream.  We are currently investigating this and other instances of "light black" as a separate phenomenon.)

 

 

A black mare which produced a buckskin when bred to a bay stallion,
indicating that she is a "smoky black",  so her slightly off-black color
is caused by the action of one cream gene on solid black.  (E_aaCrcr)

 

          When two cream genes are present, the black coloration of the mane and tail are lightened to a pale gold or orange color, and the eyes are changed from normal brown to blue.

 

                                                                   

 

                                           Smoky Black                                                          Smoky Cream

 

 

          When two copies of the cream gene are present, the cream gene reaches full affect.   The effect of the cream gene on black horses may be the most difficult to understand, as when one copy of the Cream gene is present there may not appear to be any change to color, but when two copies are present the change is dramatic.  Usually the body color of a smoky cream is a bit darker than with the perlino or cremello, which would be expected, since the black body color is being doubly diluted rather than the red.

 

Also see

 

http://www.horsecolors.us/dilutions/cream/cream_chart.htm

 

and

 

http://www.horsecolors.us/dilutions/cream/cream_foal_chart.htm

 

 

... for more insight into the cream colors and their inheritance.  The second chart was created before the brown (At) gene was isolated

 

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