UNDERSTANDING EQUINE BEHAVIOR

Instructor:  Dr. Jennifer Williams

 

 

Lesson Ten

 

Behavior Research

 

 

          I am presenting information on behavior research in Lesson 10.  I’ll give you information about the scientific method and different types of research.  I’ll also summarize individual studies and what they tell us about horse behavior.  I think keeping an eye on new research is important, even if you aren’t planning to go into a research, teaching or an academic field because behavior research helps us better understand how our horses think, why they behave the way they do, and how to train them.  If you stay up to date on the most recent equine behavior research, you’ll have an edge over other horse professionals and enthusiasts who ignore it.

 

 

Previous Lesson Summary

          Before jumping into equine research, I want to review the topics from Lesson 9:  Welfare, Ethics and Donkey and Mule Behavior and Training. 

 

          The topic of Equine Welfare covers the care and health of horses and other equines.  Horses should be healthy, comfortable, well nourished, able to express normal behavior and free from pain and fear.  We looked at the American Veterinary Medical Association’s guidelines for assessing welfare as well as the Five Freedoms of Animal Welfare.  These are both good guidelines to use when assessing the welfare of horses in your care.  The Five Freedoms state that animals should have freedom (1) from hunger and thirst, (2) from discomfort, (3) from pain, injury and disease, (4) to express normal behavior, and (5) from fear and distress.  When running your own horse-related business or housing your own horses, consider how you can apply these Five Freedoms to give your horse the best possible welfare.

 

          Additionally, countries, states/provinces, and cities/towns may have laws governing animal welfare.  It is your job as an animal owner or care-taker to know and follow these laws.  Failure to do so can result in forfeiture of your animals, fines, criminal charges and/or jail time.

 

          We also discussed the difference between animal rights and animal welfare.  The basic differences are that the animal rights movement believes that animals have the same rights as humans and should not be used or exploited in any way.  They do not believe in eating meat or dairy products, using animal fur or fiber in clothing, animal testing, or using animals for entertainment, relaxation or competition.  The animal rights movement does not believe in keeping pets or working animals.  The animal rights movement does not believe you should own horses or use them for riding, driving, racing, breeding, working or anything else.    The animal welfare movement, however, believes that animals can be used by humans as long as we uphold our responsibility to treat them humanely and fairly.  The animal welfare movement believes in outlining laws for the humane care of animals and assisting with the enforcement of those laws.

 

          In Lesson 9, we also discussed Ethics in the horse industry.  These are the moral principles that govern an individual’s or group’s behavior.  Ethics are not written into the law, although different breed or show/competition associations may have their own ethical guidelines that they expect their members to uphold.   There are several things you can do to be an ethical horseman: 

 

1.   Surround yourself with ethical professionals.

2.   Continue your education.

3.   Avoid gossip.

4.   Project a professional appearance and attitude.

5.   Give back to the horse industry.

6.   Do what you say you will do.

7.   Be up front about your expectations.

 

We also covered Donkey and Mule behavior and training in Lesson 9.  A lot of horsemen and horsewomen underestimate donkeys and mules.  They tend to think of them as stubborn, dumb horses with long ears.  But the reality is that donkeys and mules can do anything a horse can do, and they are even better than horses at some jobs!  There are shows for donkeys and mules in which they’re shown in halter and showmanship, ridden in pleasure classes, shown over jumps and driven.  There are pulling competitions and driving competitions, and both donkeys and mules can make excellent pack animals.  They’re sure-footed trail animals, too, and many people prefer trail riding on a mule or donkey over riding a horse. 

 

Both donkeys and mules come in many sizes from miniature (small) to mammoth (very large, similar to a draft horse in size) and many colors. 

 

Donkeys and mules have several characteristics that set them apart from horses.  They’re often more likely to stand and fight something rather than flee (like a horse), and they’re very contemplative:  trying to work out problems and find solutions on their own.  They also don’t move away from pressure like a horse:  you can make a horse run circles in a round pen by putting mental pressure on him from behind. A donkey or mule may run a few laps and then stop, watch you and wonder what you are trying to do. Finally, they both have a strong sense of self-preservation.  They may refuse to move forward if they feel a situation is unsafe.  This makes them awesome trail mounts as they’ll keep you and them safe, but it can make training them a challenge.

 

When it comes to training, donkeys and mules both do best with positive reinforcement:  operant conditioning with positive reinforcement, shaping, and clicker training.  Most of them genuinely like to please their handler and will work hard when they understand what you want from them.

 

Training a donkey or mule can be a challenge, but they’re fun animals who really seem to love attention. 

 

 

Equine Behavior Research

 

          Research into equine behavior and learning is conducted at universities around the world by veterinarians, professors, researchers and students working on advanced degrees (Masters and Doctorates).  The research aims to examine why horses behave the way they do or how they learn particular tasks.  Even if you aren’t planning on going into a research field, I think it is important to understand how research is conducted.  This means you’ll be able to evaluate studies you read about and determine how (or if!) they apply to your program.  It is also important to stay up to date on the newest in equine research, as it can help you improve your training, housing and handling programs and make you a better horseman or horsewoman. 

 

Although we won’t discuss nutrition, lameness or disease research in this class, understanding how research works in general can help you better interpret those studies and understand how they affect the horses in your care.

 

          There are two basic types of research studies:  observational studies and designed experiments.  In observational studies, also called descriptive research, the researcher watches horses and notes their behavior.  For example, a researcher may watch a group of feral horses and identify how much time the herd stallion spends walking in one hour.  These types of studies don’t attempt to explain why something happens, they only tell us what happen. 

 

In the example above, the researcher won’t tell you that the stallion was walking because he wanted to breed a mare.  He/she would only tell you how much time the stallion spent walking in the hour he was observed.  This type of research is important because it helps us know what horses do in various situations. You can use that information to help you determine whether behavior in your own herd is normal or abnormal.

 

          Designed experiments are much more structured research in which many variables may be controlled or manipulated, data is collected and cause and effect may be demonstrated. 

 

          Researchers employ the scientific method in order to design and conduct research.  The scientific method consists of several steps:

 

1)   Ask a question

2)   Create a hypothesis

3)   Make predictions

4)   Test the hypothesis

5)   Analyze your data and make conclusions

6)   Tell others about your results

 

I’m going to use a research project of mine to help explain how you use the scientific method to conduct research.  I performed an experiment from 1999 to 2000 on imprint training in foals. 

 

1)   Ask a question:  My question was:  Does imprint training a newborn foal result in a foal that is easier to handle throughout life?  I talked to horse owners and equine professionals who were fans of imprint training and those who disliked it to get their opinions and hear about their experiences. I also reviewed the imprint training method promoted by Dr. Miller in his book Imprint Training of the Newborn Foal.

 

2)   Create a hypothesis:  Your hypothesis is your theory.  It describes what your research is trying to prove.  My hypothesis was:  If I imprint trained foals at birth, 6 hours, 12 hours, 24 hours, and 48 hours after birth, then they will be easier to handle and train at six months of age than other foals.

 

The null hypothesis says that there are no differences in your test groups.  For my study, the null hypothesis is that There is no difference in behavior for foals who were imprint trained at birth, 6, 12, 24, and 48 hours after birth and for foals who received no imprint training.

 

3)   Make predictions:  My predictions were that the foals who were imprint trained at birth, 6 hours, 12 hours, 24 hours, and 48 hours after birth would have lower heart rates and better behavior than non-imprint trained foals when exposed to the same things they experienced during imprint training at six months of age.

 

4)   Test the hypothesis:  I designed and carried out an experiment to test the hypothesis.  Half the foals in our group were assigned to a test group in which they received imprint training at birth, 6 hours, 12 hours, 24 hours, and 48 hours after birth and others were assigned to a control group that did not receive imprint training. 

 

The foals in the test group received imprint training, and those foals as well as those in the control group were put out on pasture and were not handled again until six months of age.  At six months of age, all foals were caught, removed from their dams, and put into individual pens.  I and several assistants visited the farm.  We timed how long it took to catch each foal. Next we attached a heart rate monitor to each foal.  We then exposed the foals to the same things they had been exposed to during imprint training (being touched all over, having their feet picked up, etc.).  We measured their heart rate during each item as well as how long it took to complete each item.  We also exposed them to one novel thing, an umbrella being opened, and recorded their heart rate.

 

5)   Analyze your data and make conclusions:  Once I was done gathering data, I used statistical analysis to compare the heart rates and length of time per item for the test group (foals who received imprint training) and the control group (foals who did not receive imprint training).  I did get help on the statistics as they can be tough to perform!  Based on the statistical analysis, I discovered that there was no significant difference in the heart rates or length of time it took to complete each item between the test and control groups.  In our study, imprint training did not appear to lead to foals that were easier than non-imprint trained foals to handle at six months of age.

 

6)   Tell others about your results:  I took all of the information we gathered and our conclusions and wrote a scientific article which was published in the Equine Veterinary Journal.

 

If you aren’t conducting your own research, the part you’ll be most concerned with is step six:  the part where researchers share their results with others.  Sometimes this is done at scientific conferences, but it is often done in articles published in respected scientific journals.  Those articles consist of five parts:

 

1)   Abstract:  This is a brief summary of the research.  It generally explains why the research was done, how it was done, and outlines the general conclusions of the researchers.

2)   Introduction:  This gives a more in-depth introduction to the subject.  It explains what the researcher is studying and reviews related studies to describe what’s been discovered in the past.

3)   Materials (also called Materials and Methods):  This part of the article describes how the research was conducted.  It includes a description of the treatment group(s) and control group, describes any equipment that was used, gives a detailed description of the steps performed during the research and how data was collected, and discusses what statistics were used to analyze the data.

4)   Results:  This part of the article describes what data was collected, and it often includes tables or graphs.  It then discusses the statistical tests performed and their results.

5)   Discussion:  This part of the article discusses the results.  In this part, the authors may draw conclusions about what the statistics mean and will discuss whether or not they proved or disproved their hypothesis.  The authors may include information on further research that’s needed. 

 

Several horse magazines include brief summaries of recent research in behavior, health, training, but they don’t have the space to go into any great detail, so it is best to look for the actual study so you can understand what was done and evaluate why it is or isn’t applicable to your own horses or program.  There are a few internet sites that give either partial or full access to journal articles:

 

·         Pubmed:  http://www.ncbi.nlm.nih.gov/pubmed/

·         Google Scholar:  http://scholar.google.com/

·         AGRICOLA:  http://www.ebscohost.com/academic/agricola

 

When looking at published research, you will likely first see a citation somewhere.  The citation tells who conducted the study, when it was published, its title, the journal it is in, and how to find it.  For instance, using my imprint training study:

 

J.L. Williams, T.H. Friend, M.N. Collins, M.J. Toscano, A. Sisto-Burt, and C.H. Nevill. 2003.  The effects of an imprint training procedure conducted at birth on the reactions of foals at six months of age.  Equine Veterinary Journal.  35(2).

 

Translating the citation:  The study was conducted by J.L. Williams (me), T.H. Friend, M.N. Collins, M.J. Toscano, A. Sisto-Burt, and C.H. Nevill (many studies have a group of people working on them) and published in 2003.  The title of the article is “The effects of an imprint training procedure conducted at birth on the reactions of foals at six months of age” and it was published in the Equine Veterinary Journal.

 

Now, let’s look at some published research.  We’ll discuss what the researchers found and how you might use that to refine your own training/handling/horse keeping program.  We’ll start with the three studies I’m the most familiar with – because I am the one who conducted them!

 

Citation:  J.L. Williams, T.H. Friend, M.N. Collins, M.J. Toscano, A. Sisto-Burt, and C.H. Nevill. 2003.  The effects of an imprint training procedure conducted at birth on the reactions of foals at six months of age.  Equine Veterinary Journal.  35(2).

 

          We discussed a part of this this study a bit above.  When we conducted it, we used 131 foals born at the same facility.  The foals were divided into several treatment groups that received standard imprint training:

 

1)   Not at all

2)   At birth, 12, 24, and 48 hours after birth

3)   At birth only

4)   At 12 hours after birth only

5)   At 24 hours after birth only

6)   At 48 hours after birth only

 

When the foals were approximately 48 hours old, they were turned out on pasture with their dams and were not handled again until they were six months old.  At that time, they were brought in and weaned and then tested.  Testing included timing how long it took to catch and halter each foal.  We then attached a heart rate monitor to each foal and recorded his/her highest heart rate while being exposed to each item on the imprint training procedure and one new item.  We also timed how long it took to do each thing.

 

I then used statistical analysis to compare the heart rate and length of time for each item.  I found no difference in the heart rate or length of time to conduct each item for any of the treatment groups, and I concluded that imprint training did not create foals who were easier to handle or less reactive at six months of age. 

 

Since I conducted the research, I know we did this study to see if horse owners could invest some time imprint training early on in order to save time handling foals later.  Obviously, this didn’t work. 

 

The practical aspect for horse owners/trainers:   This doesn’t mean all imprint training is bad or useless, but it does mean that you need to follow imprint training up with good, consistent handling in order to have foals that are easier to handle later in life.       

 

Citation:  J. L. Williams, T. H. Friend, M. J. Toscano, M. N. Collins, A. Sisto-Burt and C. H. Nevill.  2002. The effects of early training sessions on the reactions of foals at 1, 2, and 3 months of age.  Applied Animal Behaviour Science.  77(2): 105-115.

 

          In this study, we used the same imprint training procedure as the above study, but foals were either imprint trained at birth, 6, 12, 24, and 48 hours after birth or not at all (only two treatment groups).  We then retested the foals when they were 1, 2, and 3 months of age by measuring their heart rate, how long it took to conduct each item on the imprint training procedure, and assessing their behavior.  47 foals were tested at 1 month of age, 20 at two months, and nine at three months.  Imprint trained foals tended to have lower heart rates and require less time to complete each item on the imprint training procedure at one and two months of age, but there was no significant difference between control foals (no imprint training) and imprint trained foals at three months of age.

 

          The practical aspect for horse owners/trainers:  This may indicate that consistent handling is more important than an early imprint training procedure.  Again, it doesn’t mean imprint training is bad or will have a bad effect on the foals, it simply means you need to follow-up with good, consistent handling (and if you chose not to imprint train, you aren’t harming your foals!).

 


Citation;  J.L. Williams, T.H. Friend, C.H. Nevill, and G. Archer. 2004.  The efficacy of a secondary reinforcer (clicker) during acquisition and extinction of an operant task in horses. Applied Animal Behaviour Science. 

 

In this study, we divided the horses into six groups:

 

1)   Primary reinforcer only, continuous schedule of reinforcement, no reinforce during extinction

2)   Primary reinforcer only, variable schedule of reinforcement, no reinforce during extinction

3)   Secondary and Primary reinforcers, continuous schedule of reinforcement, no reinforce during extinction

4)   Secondary and Primary reinforcers, continuous schedule of reinforcement, secondary reinforce during extinction

5)   Secondary and Primary reinforcers, variable schedule of reinforcement, no reinforce during extinction

6)   Secondary and Primary reinforcers, variable schedule of reinforcement, secondary reinforce during extinction

 

We used several treatments so we could see if there were differences between operant conditioning alone and clicker training (operant conditioning with the use of a secondary reinforcer).  We also wanted to examine differences with a continuous versus variable ratio schedule of reinforcement, and we wanted to know if extinction occurred more quickly when the clicker was present at extinction than when it was not present.

 

We then trained each horse to touch their nose to a cone attached to a stand in front of them when given the command “touch”.  Once the horse was consistently touching the cone when we issued the command “touch”, we stopped administering a primary reinforcer and stopped using a secondary reinforcer in some treatments and measured how long it took the horse to stop responding to the touch command.

 

We found no differences in the number of times it took the horses to learn to touch the cone consistently and the length of time for them to stop touching the cone during extinction.  I concluded the clicker training was no more or less effective than training using only a primary reinforce (in this case that was food) in the length of time it took for a horse to learn an operant task or the length of time it took him to stop performing it once he was no longer receiving primary reinforcement.

 

The practical aspect for horse owners/trainers:  Clicker training won’t train horses more quickly, but it does make sense for some people.  I have used clicker training myself to teach a wild horse to allow me to approach, touch him, and halter him. 

         

Citation:  H. Caanitz, L. O’Leary, K. Houpt, K. Petersson, and H. Hintz.  1991.  Effect of exercise on equine behavior.  Applied Animal Behaviour Science.  31(1-2): 1-12.

 

          This study compared the behavior of six horses on days when they were exercised hard on a treadmill versus days when they were not exercised.  The researchers observed the horses’ behavior in their stalls and found that horses spent more time drinking and less time resting on days when they had exercised.  The amount of time spent eating, urinating, defecating and walking was the same on days with exercise and those without exercise.

 

          The practical aspect for horse owners/trainers:  While this research wouldn’t affect your training program, it is good to know what’s normal for horses in different environments.  That will allow you to pick up on subtle differences that may indicate a horse is stressed or ill.  For example, if one of your horses stops drinking and begins pacing after exercise, that is abnormal and you should check to make sure he’s not suffering colic, is sick, or stressed.

 

Citation:  Y. Momozawa, T. Ono, F. Sato, T. Kikusui, Y. Takeuchi, Y. Mori, R. Kusunose. 2003.  Assessment of equine temperament by a questionnaire survey to caretakers and evaluation of its reliability by simultaneous behavior test.  Applied Animal Behavior Science.  64(2):  127-138.

 

          The researchers asked the caretakers of 86 riding horses to rate the horses’ temperaments during ordinary care and daily training.  Then the researchers put each horse, one at a time, in an unfamiliar indoor arena where there were two balloons that slowly revolved.  The researchers measured the horses’ responses by noting changes in behavior and heart rate. 

 

          Researchers then compared the results of the surveys with the results of the balloon experiment.  They found that horses who were rated as anxious by their caretakers were more likely to have elevated heart rates and defecate more frequently (both signs of stress) during the balloon test. 

 

          The practical aspect for horse owners/trainers:   This research indicates that 1) caretakers can accurately assess the temperament of the horse, and 2) that horses that are more anxious in daily handling and training are also more likely to show signs of stress and fear in new settings.

 

Citation:  J.L. Kamm, D.A. Henderickson.  2007.  Clients’ perspectives on the effects of laparoscopic ovariectomy on equine behavior and medical problems.  Journal of Equine Veterinary Science.  27(10): 435-438.

 

          35 mares with behavioral or medical problems were treated with ovariectomies (their ovaries were removed).  Later, the researchers contacted the mares’ owners with a series of questions regarding the mares’ behavior after the procedure. 

 

          Aggression improved for 86% of the mares, grouchiness or disagreeable behavior improved for 81% of the mares, excitability improved in 75% of the mares, kicking and biting improved in 73% of the mares, training problems were reduced for 72% of the mares and problems with other horses improved in 64% of the mares.  Frequent urination improved in 64% of the mares, but there was no significant improvement in cycle-related laminitis.

 

          The practical aspect for horse owners/trainers:   Some mares are very difficult to deal with, especially when they cycle.  If they are not good breeding candidates, you may consider having their ovaries removed.  It does not improve behavior and training in all cases, but it does appear to frequently improve several problems.

 

Citation:  C.R. Heleski, A.C. Shelle, B.D. Nielsen, A.J. Zanella.  2002.  Influence of housing on weanling horse behavior and subsequent welfare.  Applied Animal Behavior Science.  78(2-4):  291-302

 

          Foals in this study were divided into two groups:  those weaned individually and housed in box stalls and those weaned in small groups and housed in paddocks.  After being placed in the stalls or paddocks, foals were observed two days a week for 56 days.  Weanlings housed in paddocks in groups of three behaved like feral horses:  spending their time moving around and attempting to graze.  Those housed in stalls showed more abnormal behaviors:  kicking at the stall wall, licking or chewing the stall wall, pawing and bucking. 

 

          The practical aspect for horse owners/trainers:   Horses who display normal, natural behaviors appear to be horses that are experiencing less stress.  So weaning foals together and keeping them outside where they can move around appears to be healthier for foals. 

 

Citation:  J.W. Christensen, M. Beekmans, M. van Dlum, M. Vandierendonck.  2014.  Effects of hyperflexion on acute stress responses in ridden dressage horses.  Physiology & Behavior.

 

          In this study, fifteen dressage horses were ridden for 10 minutes in a competition frame, hyperflexed frame, or loose frame.  The researchers measured heart rate, heart rate variability, behavior, rein tension, and cortisol levels. 

 

They found that there were no differences in heart rate or heart rate variability amongst the three treatment groups.  However, cortisol levels (a measure of stress) were higher in horses ridden in the hpyerflexed frame than in the other two treatments.  Behavior measurements, including head movements, also indicated increased stress in horses ridden in a hyperflexed frame.

 

The practical aspect for horse owners/trainers:   The use of hyperflexion when training dressage horses has recently come under suspicion.  Many opponents believe it leads to stress and physical discomfort and should be abandoned.  However researchers have just now begun studying its effects on the horses.  This study did indicate that the horse experiences additional stress and may experience discomfort, and I believe more research should be done. 

 

Citation:  K.E. Holcomb, C.B. Tucker, C.L. Stull.  2014.  Preference of domestic horses for shade in a hot, sunny environment.  Journal of Animal Science. 

 

          Researchers in this study observed 12 horses that were kept in individual pens.  Half of each pen was covered by a structure that provided shade.  They then measured the horses’ rectal temperatures, respiration rate, and skin temperatures.  They also assigned a “sweat score” based on the amount of observable sweating.  They also measured behavior:  how far the horse was from the shed, time spent walking, time spent foraging, and time spent standing. 

 

          Researchers found that the horses were more likely to be located in the shade right before and during the hottest temperatures of the day. 

 

The practical aspect for horse owners/trainers:   This research supports the recommendation to provide shade to horses, particularly in hot, sunny climates.

 

Assignment

          Use one of the search methods described in this lesson to locate two research articles about equine behavior (these articles must be published in scientific journals, not horse magazines).  Provide the citation for each article, the search method used to find it, describe what the researchers did and their results, and describe the practical application for horse owners.

 

          Please send essay to Dr. Williams at equinebehaviorinstructor@gmail.com

          Be sure to include your full name and email address on the document – not just in the email.

 

Click Here To Take Quiz