Equine Coat Color Genetics

 

Lesson Seven

 

Patterns of White

Part One – General Overview and Tobiano

 

            Last lesson we talked about genes which add white hairs to the horse’s coat, usually mixed in rather uniformly, without affecting the pigment in the skin underneath.  The other category of “white adding” genes are the kind that cause white areas of any size with depigmented skin underneath.  There are many different genes that do this.  One important thing to keep in mind is that, like grey and roan, these patterns are superimposed on top of whatever color the horse would have been without them.  On horses with large amounts of white spotting, it can be hard to tell what the base color is, but every horse has one. 

If a grey horse has white pattern markings, the colored areas will gradually fade as the horse goes grey, until the whole horse is white, making it hard to tell where the spots were.  But the skin will remain pigmented under the grey parts, so the spots can be seen when the horse is wet or shaved.

Broadly speaking, white patterns are broken down into three general types:  White markings, pinto type spotting, and appaloosa type spotting. 

“Regular” white markings such as stars, snips, and socks occur in almost every breed and the genetic mechanism is unknown.  Some of the known spotting genes do cause markings such as these when minimally expressed, but the vast majority of horses with ordinary markings do not carry a known spotting gene.  There are a wide variety of names for the various types of markings.  Most registries have their own terminology.  The Thoroughbred registry has one of the largest vocabularies for describing white markings.  You can see them here:

https://www.registry.jockeyclub.com/registry.cfm?page=headmark&CFID=19213068&CFTOKEN=97832309cc1492da-C8C286B2-5056-BE2F-781D7DCE2295AB86

and here:

https://www.registry.jockeyclub.com/registry.cfm?page=legmark&CFID=19213068&CFTOKEN=97832309cc1492da-C8C286B2-5056-BE2F-781D7DCE2295AB86

(If those links no longer work, you can search the jockeyclub.com site for “registration” and “how to identify”.)  Most every other major breed registry has a similar guide on their website.  We won’t go through all the names for all the various types of markings, since they vary somewhat from one breed to another and from one place to another, and since the genetics of them are unknown at this time. 

Appaloosa type spotting is unique and will have its own section.

The third broad category is generally called “pinto” spots but has many other names as well.  In Europe, the appaloosa type are called “spotted” and the pinto type are called “coloured”.  It has been called “broken” coated (as in, the dark areas are “broken up” by white areas) in some places.  In England in the past, black and white pintos were called “piebald” and any other color and white was called “skewbald”.  (This terminology persists to some degree in some places, but is discouraged since it tells you nothing about the actual pattern involved.)  Another term you may come across is “particolored”.  In America they are commonly called paint or pinto.

All of the color patterning genes can occur singularly or in any combination.  A horse carrying both appaloosa and pinto genes is referred to as a “pintaloosa and is unregisterable by the American Paint Horse Association,  Pinto Horse Association or Appaloosa associations.  These breeds are rarely crossed.  However, Miniature horses do carry all these genes, and pintaloosas are welcome in their registry, so there are some out there.

One important note about white spotting genes.  They do not control “how much” white the horse has, only the “location and character” -- where on the body it will be, and what it will look like.  Horses with more than one kind of pattern gene tend to have more white, but not always.  It is poorly understood what makes one horse have much more white than another, and it may be to some extent environmental or pure chance, since clones do not have exactly identical markings.  However a general tendency toward lots of white or very little does seem to run in families to some extent, so probably has a genetic basis.  This explains how some of the pinto patterns were thought to be recessive for many years.  It could “hide” on a minimally-marked horse (one that does in fact have a pinto gene, but it looks like ordinary markings) and then when crossed with a horse that happens to have genes for “lots of white” – voila!  A loud, body-spotted pinto results. 

 

The Paint/Pinto Coat Patterns

First let’s discuss the terms “Pinto” and “Paint” and mark the difference in the use of these terms.  When not capitalized, they refer to a horse of any breed with white body spots of any pattern.  Paint is more common in the West, and pinto in the east, but “pinto” is a general term for any white spotted horse.  When capitalized, it is generally understood to mean a horse that is registered in that registry.  The Pinto Horse Association (PtHA) is a registry for patterned horses of many breeds, with the exception of any Appaloosa, draft or mule breeding and/or characteristics.  More info:  http://www.pinto.org/index.php/about-us/the-breed  The American Paint Horse Association (APHA) is a “stock horse” breed which only includes horses of APHA,  Quarter Horse, or Thoroughbred blood. More info:  http://apha.com/association/history/ 

These registries are widespread and popular, and have worked to increase the horse-owning public’s understanding of pinto patterns.  Unfortunately, when it comes to genetics, they are both somewhat “behind the times”.  The Paint and Pinto registries both break down patterns into two basic types:  Tobiano and Overo.  (A horse with both patterns is called “Tovero”.)  Over the years it has been found that there are many more white-spotting genes than just two.  Tobiano remains separate and is caused by only one gene, but the “Overo” category really just means “anything other than tobiano” now, and there are many genes in that category.  We will leave them for next time, and focus on just Tobiano this time.

 

 What a Tobiano Looks Like

The pattern of a tobiano (pronounced tow-bee-yah-no) is evident from the moment of birth. The white areas characteristically cross over the back and produce four white legs at least below the knees and the hocks.  Tobiano has often been said to look like “a white horse with dark spots” (as opposed to overo, which is opposite), however, this is rather a matter of opinion.

 

joe   

Typical tobiano features - white legs, white crossing over the back, a neck shield and a dark head.

 

The dark (base color) body spots have smooth and distinct edges which are usually rounded rather than jagged.  The chest may be dark creating a "shield" and at least one flank is usually colored. The head of a tobiano is usually dark, and Tobiano does not put white on the face.  (They may have regular white markings like any non-pinto horse, of course.)  This is true even in mostly white tobianos, as the dark color of the head is the last place to be covered up by white.  Tobiano does not cause blue eyes, so the eyes are usually dark, although blue eyes can pop up in rare cases in any horse.  The tail may be two-toned, which is rarely seen with other patterns.

Tobiano markings may range from an almost solid white horse with color only on the ears to a almost solid animal with white only on the legs.  The APHA shows typical Tobiano patterns as:

Images of Tobiano coat patterns.  The consistency are the white legs and a dark head.

 

How the Tobiano Gene Works

The tobiano spotting pattern is caused by a large chromosomal inversion (you could think of part of the chromosome being upside down) in the “KIT” complex on chromosome 3.  The tobiano gene is dominant so is called “T”. (Sometimes you will see it written as “To”).  A heterozygous tobiano would be Tt.   Fifty percent of the foals produced from a heterozygous tobiano should have the tobiano pattern.

To the delight of Paint Horse breeders, the homozygous tobiano exists.  Homozygous tobianos result from the mating of two tobiano parents and, per a Punnet Square, one out of every four foals produced by two heterozygous tobiano parents should be a homozygous tobiano.

 

 

Having two copies of the tobiano gene does not increase the amount of white on the horse, but, the individuals carrying this genotype can often be identified by the small dark hairs scattered in clusters in the white areas of its coat. Most, but not all homozygous tobianos have these "ink spots" or "paw prints", and most, but not all, heterozygous tobianos do not have them.  There are documented exceptions, though, so it’s not a “for sure” thing. 

 

CAN DO FULL COLOR (APHA)

A homozygous tobiano stallion with characteristic ink spots.

 

Homozygous tobiano are prized for their ability to produce tobiano offsprings. Statistically, every foal produced from the mating should receive one copy of the dominant tobiano gene "T", thus creating a tobiano.

 

Sonny Double Dee (APHA)

Homozygous tobiano mare with paw prints on hip.

 

Minimal White Tobianos

Since white pattern genes such as Tobiano do not determine “how much” white the horse will have, occasionally there will be a tobiano that does not have any white spots on its body.  These horses are often referred to as “crypto tobianos” or “slipped tobianos(Think of it as the “white” has slipped down the body to the lower legs.)  For these horses, the tendency of the tobiano to have a dark head and white legs holds true even if the white legs are no more than white pasterns or fetlocks. Horses with ordinary markings tend to have similar amounts of white on both legs and head.  A small star and a small sock or two, or a large blaze with four stockings, for example.  This combination of white legs and a dark face is highly likely to be a tobiano.

 

An example of a “Slipped Tobiano”. 

Notice the lack of Pinto markings on the body and white limited to the legs only

 

The Paint and Pinto registries have strict rules about minimum sized body spots that make a horse eligible for registration.  A horse without body spots is called “Breeding Stock” or “Solid” even if they actually have a pinto gene.  Even though horses such as these are registered as “Solid”, they are, in fact, tobianos and do pass on the dominant Tobiano gene 50% of the time (or 100% of the time if they are homozygous, TT).

 

Testing for the Tobiano Gene

The inversion associated with the Tobiano pattern was identified by researchers at the University of Kentucky.  Since the gene has now been isolated, there is a DNA test, available at most labs.  One example:  http://animalgenetics.us/Equine/Coat_Color/Tobiano.asp

 

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